Cell lines are widely used in laboratories for in vitro experiments, especially for investigating abnormal hallmarks in cancer cells and identifying therapeutic targets. To achieve a representation of intracancer heterogeneity, several laboratories, have established cell line collections. However, the establishment and maintenance of such collections significantly increase the risk of cross-contamination and misidentification of cell lines, leading to the publication of false data/interpretation. A rapid and low-cost method for re-identification after each thawing of cells is required (typically carried out 4 times per year), as it is for mycoplasma detection. A simple and low cost method involving human leukocyte antigen (HLA) typing and phenotyping could be used for routine re-authentication using flow cytometry.